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Anti-SMAD2

Rabbit Polyclonal Antibody
Catalog No. S11-63R


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Catalog No. Pack Size Price (USD)
S11-63R-100 100 ug $347
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Overview:

SMADs are essential intracellular components for the signal transduction of TGFβ family members. SMAD2 is an intracellular mediator of TGFβ family and activin type 1 receptor (1). SMAD2 mediate TGFβ signaling to regulate cell growth and differentiation. SMAD2 is released from cytoplasmic retention by TGFβ receptor-mediated phosphorylation. The phosphorylated SMAD2 then forms a heterodimeric complex with SMAD4, and this complex translocates from cytoplasm into nucleus. By interacting with DNA-binding proteins, SMAD2 complexes then positively or negatively regulate the transcription of target genes. Inactivating mutations in SMAD2 have been found in various cancers (2).

References:


1. Masayuki, F. et al: Identification and Characterization of Constitutively Active Smad2 Mutants: Evaluation of Formation of Smad Complex and Subcellular Distribution. Molecular Endocrinol. 2000; 14 (10): 1583-1591.

2. Eppert, K. et al: MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell. 1996; 86: 543-552.


Specificity:

Recognizes the SMAD2 protein


Cross Reactivity:

Western blot human SMAD2

SMAD2 from other species may also be detectable.


Host :

Rabbit


Immunogen:

Synthetic peptide corresponding to amino acids 218-222 of human SMAD2


Purification:

Affinity chromatography


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative Western blot with Anti-SMAD2 (1:1000) using 15ng of GST-tagged human recombinant SMAD1, SMAD2, SMAD3, SAMD4 and SMAD5.


Sample Data:

Representative Western blot with Anti-SMAD2 (1:500) using 20 μg, 40 μg and 65 μg of Jurkat cell lysate.



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

AKT/PKB Pathway, Cancer, Cell Cycle, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, WNT Signaling


RELATED PRODUCTS


  SMAD2 Protein, S11-30G

  SMAD4 Protein, S13-30G

  SMAD1 Protein, S10-30G

  SMAD3 Protein, S12-30G


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