Ubiquitin Enzymes

Ubiquitin Enzymes

Protein modifications by ubiquitin (Ub) or ubiquitin-like proteins (UBLs) participate in many critical cellular processes such as cell-cycle regulation, DNA repair, oncogenesis, antiviral pathways and most notably, proteasomal degradation of target proteins. Ubiquitination and modification by UBLs share a similar catalytic cascade which requires the sequential action of three classes of enzymes: E1 activating enzymes, E2 conjugating enzymes, and E3 ligases. Research has linked dysregulation of the Ub/UBLs modification system to numerous diseases including cancer, immunological disorders, and neurodegeneration. Thus, the high substrate specificity provided by combinations of over 30 E2’s and over 600 E3’s makes these enzymes emerging drug targets.

In response to growing market demand, SignalChem has developed an extensive array of products encompassing ubiquitin proteins and active enzymes, Ub/UBL modifiers, and substrates in the ubiquitination, SUMOylation, ISGylation and NEDDylation processes. Using Promega’s AMP-Glo™ technology and an optimized assay protocol, we have identified and validated a variety of functional combinations of the enzyme components. With the established protocol, each enzyme in the catalytic cascade has been assessed for their activity towards the generation of free AMP. In addition, inhibition profiles of the ubiquitinating enzymes have been obtained using the assay system, further demonstrating their potential to be used in high-throughput screening to identify lead compounds for drug discovery and development programs.

Home     >     Enzymes
Name Catalog Number
BIRC3, Active B280-380G
BIRC7, Active B281-380G
HERC4, Active H265-381G
MGRN1, Active M287-380G
RanBP2, Active R230-381H
RNF34 (CARP), Active R296-380G
RNF34L (CARP2), Active R297-380G
TRIM37, Active T292-380G
WWP2, Active W297-380G
CBL Protein C272-381G
FANCL Protein F289-30G
Parkin Protein P215-30G
PRPF19 Protein P270-30G
STUB1 (CHIP) Protein C268-30H
TRIM32 Protein T291-30G
WWP1 Protein W296-30G
VHL/ELOB/ELOC/CUL2/RBX1 Complex U272-380H
CRBN/DDB1 Complex U274-34H
CRBN/DDB1/CUL4A/RBX1 Complex U273-380H



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