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SMAD4 Protein

Recombinant full-length human SMAD4 was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. S13-30G


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Catalog No. Pack Size Price (USD)
S13-30G-50 50 ug $435
S13-30G-20 20 ug $215
S13-30G-BULK BULK Contact Us  


Overview:

SMAD4 is a member of the SMAD family and mediates signaling by the transforming growth factor-beta (TGFβ) superfamily and related ligands (1). TGFβ stimulation leads to phosphorylation and activation of SMAD1, SMAD2 and SMAD3, which form complexes with SMAD4 that accumulate in the nucleus and regulate transcription of target genes. SMAD signaling is negatively regulated by inhibitory SMADs and ubiquitin-mediated processes and proteasomal degradation of SMADs depend on the direct interaction of specific E3 ligases with SMADs. SMAD4 is targeted for degradation by multiple ubiquitin ligases that can simultaneously act on R-SMADs and signaling receptors. Such mechanisms of down-regulation of TGFβ signaling via degradation of SMADs may be critical for proper physiological response to this pathway (2).


Gene Aliases:

JIP, DPC4, MADH4


Genbank Number:


References:


1. Heldin, C.H. et al: TGF-beta signalling from cell membrane to nucleus through SMAD proteins". Nature, 1997, 390 (6659): 465–71.

2. Attisano, L. et al: Mads and Smads in TGF beta signalling". Curr. Opin. Cell Biol. 1998; 10 (2): 188–94.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~95 kDa



Product Datasheets



RESEARCH AREAS

AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, WNT Signaling


RELATED PRODUCTS


  SMAD2 Protein, S11-30G

  SMAD3 Protein, S12-30G

  SMAD5 Protein, S14-30G

  SMAD1 Protein, S10-30G


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