Rabbit Polyclonal Antibody
Catalog No. G13-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
G13-63R-100 | 100 ug | $455 | |
G13-63R-BULK | BULK | Contact Us |
Overview:
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis [1]. GAPDH also serves as an ideal loading control or housekeeping gene [2].
References:
1.Tarze, A, et al: GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization. Oncogene. 2007. 26 (18): 2606-2620. PMID 17072346
2.Said, HM, et al: GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol. 2007 Jun 27; 8:55. PMID 17597534
Specificity:
Recognizes only the GAPDH protein
Cross Reactivity:
Western blot GAPDH from Rabbit and Mouse
GAPDH from other species may also be detectable.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to a portion of the human G3PDH
Purification:
Affinity Chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative Western blot with Anti-GAPDH (1:1000) using 30μg of HeLa, 293, Jurkat and THP1 cell lysates.
H. Lim Sung et al., Luteolin suppresses UVB-induced photoageing by targeting JNK1 and p90RSK2 Journal of Cellular and Molecular Medicine April 2013 10.1111/jcmm.12050
ZL Tian et al., Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age International Journal of Legal Medicine January 2016 10.1007/s00414-015-1251-x
Long Zheng Ji et al., Cannabinoid receptor type 2 is time-dependently expressed during skin wound healing in mice International Journal of Legal Medicine September 2012 10.1007/s00414-012-0741-3
Shui Yu Tian et al., The cannabinoid receptor type 2 is time-dependently expressed during skeletal muscle wound healing in rats International Journal of Legal Medicine June 2010 10.1007/s00414-010-0465-1
Cheng Zheng et al., Protective Role of the Cholinergic Anti-Inflammatory Pathway in a Mouse Model of Viral Myocarditis PLoS One November 2014 10.1371/journal.pone.0112719
Ju Lin Ying et al., Characterization of the GXXXG motif in the first transmembrane segment of Japanese encephalitis virus precursor membrane (prM) protein Journal of Biomedical Science May 2010 10.1186/1423-0127-17-39
Subangari Dorasamy Mathura et al., Dihydroorotate dehydrogenase Inhibitors Target c-Myc and Arrest Melanoma, Myeloma and Lymphoma cells at S-phase J Of cancer September 2017 10.7150/jca.14835
Yan Fan Yan et al., Nicotinic acetylcholine receptor alpha7 subunit is time-dependently expressed in distinct cell types during skin wound healing in mice Histochemistry and Cell Biology April 2011 10.1007/s00418-011-0798-y
Ling Tian Zhi et al., α7nAChR is expressed in satellite cells at different myogenic status during skeletal muscle wound healing in rats Journal of Molecular Histology October 2015 10.1007/s10735-015-9641-4
Yan Fan Yan et al., Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats Journal of Molecular Histology February 2013 10.1007/s10735-012-9445-8
Zhang ST et al., Nrf1 is time-dependently expressed and distributed in the distinct cell types after trauma to skeletal muscles in rats Histology and Histopathology November 2012 10.14670/HH-28.725
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