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Anti-JNK1

Rabbit Polyclonal Antibody
Catalog No. M33-63R


For enquiry on all Isoform Specific Antibodies email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
M33-63R-25 25 ug $127
M33-63R-100 100 ug $347
M33-63R-BULK BULK Contact Us  


Overview:

JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.

References:


1. Derijard, B. et al: JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell. 1994 Mar 25;76(6):1025-37.

2. Kolodziejczyk, S M. et al: Activation of JNK1 contributes to dystrophic muscle pathogenesis. Curr Biol. 2001 Aug 21;11(16):1278-82.


Specificity:

Recognizes the JNK1 protein


Cross Reactivity:

Western blot JNK1 from human, mouse, and rat cells

JNK1 from other species may also be detectable.


Host:

Rabbit


Immunogen:

Protein Code: P45983


Purification:

Affinity purified by the peptide antigen


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Western Blot:


Representative western blot with Anti-JNK1 (1:250) using 20 ng of JNK1, JNK2, and JNK3 recombinant protein.



Product Datasheets



 KH Su et al., HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nature Cell Biology May 2016 10.1038/ncb3335

 Meter M Van et al., JNK Phosphorylates SIRT6 to Stimulate DNA Double-Strand Break Repair in Response to Oxidative Stress by Recruiting PARP1 to DNA Breaks. Cell Reports September 2016 10.1016/j.celrep.2016.08.006

 Hong Park Sun et al., IRAK4 as a Molecular Target in the Amelioration of Innate Immunity?Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid Journal of Immunology November 2014 10.4049/jimmunol.1402101

 A. Dadaa Laura et al., High CO2 Leads to Na, K-ATPase Endocytosis via c-Jun Amino-Terminal Kinase-Induced LMO7b Phosphorylation Molecular and Cellular Biology September 2015 10.1128/MCB.00813-15

 Selvaraja Nagarathinam et al., Extracellular Signal-Regulated Kinase Signaling Regulates the Opposing Roles of JUN Family Transcription Factors at ETS/AP-1 Sites and in Cell Migration Molecular and Cellular Biology January 2015 10.1128/MCB.00982-14

 Prakasam A et al., JNK1/2 regulate Bid by direct phosphorylation at Thr59 in response to ALDH1L1 Cell Death & Disease July 2014 10.1038/cddis.2014.316

 Liu Bin et al., ABL-N-induced apoptosis in human breast cancer cells is partially mediated by c-Jun NH2-terminal kinase activation Breast Cancer Research January 2010 10.1186/bcr2475

 Nakatsu Daiki et al., JNK1/2-dependent phosphorylation of angulin-1/LSR is required for the exclusive localization of angulin-1/LSR and tricellulin at tricellular contacts in EpH4 epithelial sheet Genes to Cells June 2014 10.1111/gtc.12158

 Selvaraj Nagarathinam et al., Comparison of MAPK specificity across the ETS transcription factor family identifies a high-affinity ERK interaction required for ERG function in prostate cells Cell Communcation & Signaling February 2015 10.1186/s12964-015-0089-7


RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, NfkB Pathway


RELATED PRODUCTS


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  JNK2, Active, M34-10BG


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