Recombinant human AXL (473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Catalog No. A34-11H
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A34-11H-05 | 5 ug | $226 | |
A34-11H-10 | 10 ug | $325 | |
A34-11H-BULK | BULK | Contact Us |
Overview:
AXL is a member of the receptor tyrosine kinase family which has oncogenic potential and is implicated in human myeloid leukemia (1). AXL is a member of a complex signaling network that is involved in the control of cell proliferation and differentiation. Overexpression of AXL cDNA in NIH 3T3 cells induces neoplastic transformation of these cells with the concomitant appearance of a 140kDa AXL tyrosine-phosphorylated protein (2). Expression of AXL cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the AXL protein is tyrosine phosphorylated.
Gene Aliases:
UFO, JTK11
Genbank Number:
References:
1. O'Bryan, J.P. et al: Axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase. Mol Cell Biol. 1991 Oct;11(10):5016-31.
2. Janssen, J.W. et al:, A novel putative tyrosine kinase receptor with oncogenic potential. Oncogene. 1991 Nov;6(11):2113-20.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~55 kDa
Kambayashi T et al., TAM receptors attenuate murine NK cell responses via E3 ubiquitin ligase Cbl-b
European Journal of Immunology September 2019 10.1002/eji.201948204
Zhang Zaihui et al., Discovery of novel TAM family kinase inhibitors Cancer Research April 2014 10.1158/1538-7445.AM2014-2716
Zhang Zaihui et al., Novel and selective Axl inhibitors Cancer Research April 2014 10.1158/1538-7445.AM2014-1746
Abu-Thuraiaa Afnan et al., Axl phosphorylates Elmo scaffold proteins to promote Rac activation and cell invasion Molecular and Cellular Biology October 2014 10.1128/MCB.00764-14
Cheng LC et al., AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells journal of Biomedical science November 2018 10.1186/s12929-018-0465-x
Krejci Pavel et al., NF449 Is a Novel Inhibitor of Fibroblast Growth Factor Receptor 3 (FGFR3) Signaling Active in Chondrocytes and Multiple Myeloma Cells Journal of Biological Chemistry July 2010 10.1074/jbc.M109.083626
Kosten Jonas et al., Efficient Modification of Alpha-Synuclein Serine 129 by Protein Kinase CK1 Requires Phosphorylation of Tyrosine 125 as a Priming Event ACS Chemical Neuroscience October 2014 10.1021/cn5002254
et al., Regulation of ferredoxin-catalyzed photosynthetic phosphorylations. Proceedings of the National Academy of Sciences of the United States of America December 1975
Angiogenesis, Cancer, Receptor Tyrosine Kinases
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