Recombinant full-length human LIMK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. L05-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L05-10G-05 | 5 ug | $226 | |
L05-10G-10 | 10 ug | $325 | |
L05-10G-BULK | BULK | Contact Us |
Overview:
LIMK2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain (1). LIM domains are highly conserved cysteine-rich structures that can mediate protein-protein interaction. LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and then LIMK2 in turn phosphorylates cofilin, inhibiting its actin-depolymerizing activity which contributes to Rho-induced reorganization of the actin cytoskeleton (2). LIMK2 is highly expressed in liver, colon, stomach, spleen, brain, kidney, and placenta.
Gene Aliases:
(None)
Genbank Number:
References:
1. Okano, I. et.al: Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs. J. Biol. Chem. 270: 31321-31330, 1995.
2. Maekawa, M. et.al: Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. Science 285: 895-898, 1999.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~96 kDa
There are no related publications available for this product.
Angiogenesis, Cancer, Cardiovascular Disease, Invasion/Metastasis, Ser/Thr Kinases
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