Recombinant full-length human UBA1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. U201-380G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
U201-380G-20 | 20 ug | $215 | |
U201-380G-50 | 50 ug | $435 | |
U201-380G-BULK | BULK | Contact Us |
Overview:
Ubiquitin-activating enzyme 1 (UBA1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. UBA1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Posttranslational modification by ubiquitin or ubiquitin-like proteins regulates numerous processes, including cell division, immune responses and embryonic development.
Gene Aliases:
UBE1, CTD-2522E6.1, A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1X
Genbank Number:
References:
1. Mondal, S. et al: A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510: 41-51, 2016
2. Haas, A.L. et al: Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation. J. Biol. Chem. 257(5):2543-2548, 1982
3. McGrath, J.P. et al: UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J. 10(1):227-236, 1991
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
145 kDa
Piazza R
et al., De novo UBE2A mutations are recurrently acquired during chronic myeloid leukemia progression and interfere with myeloid differentiation pathways
Haematologica February 2019 10.3324/haematol.2017.179937
et al., The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology The Journal of Biological Chemistry February 2018
Cancer, Cell Cycle, Neurobiology
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