Recombinant human full-length mutant MEK1 (K97A) was expressed by E. coli using an N-terminal His tag. This kinase-dead protein is designed for up-stream kinase assay.
Catalog No. M02-16H
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M02-16H-20 | 20 ug | $215 | |
M02-16H-50 | 50 ug | $435 | |
M02-16H-BULK | BULK | Contact Us |
Overview:
MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).
Gene Aliases:
MAP2K1, MKK1, MAPKK1, PRKMK1
Genbank Number:
References:
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.
2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~49 kDa
Spirli Carlo et al., Cyclic AMP/PKA-dependent paradoxical activation of Raf/MEK/ERK signaling in polycystin-2 defective mice treated with sorafenib Hepatology August 2012 10.1002/hep.25872
Pan C et al., Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation The Journal of Clinical Investigations August 2019 10.1172/JCI125963
Riegel K et al., RAF kinases are stabilized and required for dendritic cell differentiation and function Cell Death and Differentiation September 2019 10.1038/s41418-019-0416-4
Gan Xiaoqing et al., Different Raf Protein Kinases Mediate Different Signaling Pathways to Stimulate E3 Ligase RFFL Gene Expression in Cell Migration Regulation Journal of Biological Chemistry November 2013 10.1074/jbc.M113.477406
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
Chao; Xu, Fengrong; Niu, Yan; Wu, Yun; Sun, Jing; Peng Wang et al., Synthesis and Biological Evaluations of 3-Benzothiazol-2-yl Coumarin Derivatives as MEK1 Inhibitors Letters in Drug Design & Discovery October 2013 10.2174/15701808113109990012
et al., ARAF suppresses ERBB3 expression and metastasis in a subset of lung cancers Science Advances March 2022
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases
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