Recombinant full-length human p110 beta and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Catalog No. P28-102H
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The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 beta catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation (1). In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110 beta impedes tumorigenesis with a concomitant diminution of AKT phosphorylation (2).
p110 beta: PIK3CB, PI3K, PIK3C1, PI3Kbeta, p110-BETA,
p85 alpha: PIK3R1, GRB1, p85-ALPHA
1. Jackson, S. P. et al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
2. Jia, S.et al: Essential roles of PI(3)K-p110-beta in cell growth, metabolism and tumorigenesis. Nature 454: 776-779, 2008.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
p110 beta ~111 kDa and p85 alpha ~86 kDa
Kong Kathleen et al., The human adenovirus E4-ORF1 protein subverts discs large 1 to mediate membrane recruitment and dysregulation of phosphatidylinositol 3-kinase PLoS Pathogens May 2014 10.1371/journal.ppat.1004102
FU Rutaganira et al., Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ Journal of Medicinal Chemistry March 2016 10.1021/acs.jmedchem.5b01311
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
R Nehme et al., New in-capillary electrophoretic kinase assays to evaluate inhibitors of the PI3k/Akt/mTOR signaling pathway Analytical and Bioanalytical Chemistry May 2014 10.1007/s00216-014-7790-z
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling