Full length recombinant human CK1 gamma 3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C68-10CG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C68-10CG-05 | 5 ug | $226 | |
C68-10CG-10 | 10 ug | $325 | |
C68-10CG-BULK | BULK | Contact Us |
Overview:
CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells (1). CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17 (2). CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms.
Gene Aliases:
CSNK1G3
Genbank Number:
References:
1. Kusuda, J. et al: Cloning and chromosome mapping of the human casein kinase I gamma-3 gene (CSNK1G3). Cytogenet. Cell Genet. 83: 101-103, 1998.
2. Zemlickova, E. et al: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~73 kDa
R Chia et al., Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nature Communications December 2014 10.1038/ncomms6827
A. Basta Holly et al., Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins Journal of Virology December 2013 10.1128/JVI.03150-13
Mamais Adamantios et al., Arsenite Stress Down-regulates Phosphorylation and 14-3-3 Binding of Leucine-rich Repeat Kinase 2 (LRRK2), Promoting Self-association and Cellular Redistribution Journal of Biological Chemistry June 2014 10.1074/jbc.M113.528463
Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling
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