Recombinant full-length human HSP70 was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. H34-54G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
H34-54G-20 | 20 ug | $215 | |
H34-54G-50 | 50 ug | $435 | |
H34-54G-BULK | BULK | Contact Us |
Overview:
HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli.
Gene Aliases:
HSPA1A, HSP70-1, HSP72, HSPA1, HSPA1B. DAQB-147D11.1
Genbank Number:
References:
1. Moran, L A. et al: The major heat-shock protein (hsp70) gene family: related sequences in mouse, Drosophila, and yeast. Can J Biochem Cell Biol. 1983 Jun;61(6):488-99.
2. Pelham, H R: Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 1984 Dec 20;3(13):3095-100.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~96 kDa
Sharda Priya et al., The dual-specificity phosphatase hYVH1 interacts with Hsp70 and prevents heat-shock-induced cell death Biochemical Journal March 2009 10.1042/BJ20081484
Winssinger Nicolas et al., Assembly of PNA-Tagged Small Molecules, Peptides, and Carbohydrates onto DNA Templates: Programming the Combinatorial Pairing and Inter-ligand Distance Peptide Nucleic Acids January 2014 10.1007/978-1-62703-553-8_8
Kostenko Sergiy et al., Phosphorylation of heat shock protein 40 (Hsp40/DnaJB1) by mitogen-activated protein kinase-activated protein kinase 5 (MK5/PRAK) The International Journal of Biochemistry & Cell Biology February 2014 10.1016/j.biocel.2013.11.004
Cancer, Cellular Stress, p38 Pathway
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