Recombinant full-length human CDK4 was expressed in E. coli cells using a N-terminal GST tag.
Catalog No. C31-14G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C31-14G-20 | 20 ug | $215 | |
C31-14G-50 | 50 ug | $435 | |
C31-14G-BULK | BULK | Contact Us |
Overview:
CDK4 is a member of the cyclin-dependent protein kinases and is involved in the control of cell proliferation during the G1 phase of the cell cycle. CDK4 forms a complex with the D-type cyclins which regulate its activity. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. Pharmacologic inactivation of CDC37/HSP90 function leads to reduced stability of CDK4. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).
Gene Aliases:
CMM3, MGC14458, PSK-J3
Genbank Number:
References:
1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.
2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~57 kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Rizzolio F et al., Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1 Cell Death & Differentiation July 2012 10.1038/cdd.2011.202
Sanphui Priyankar et al., Efficacy of Cyclin Dependent Kinase 4 Inhibitors as Potent Neuroprotective Agents against Insults Relevant to Alzheimer?s Disease PLoS One November 2013 10.1371/journal.pone.0078842
Schiappacassi Monica et al., Role of T198 Modification in the Regulation of p27Kip1 Protein Stability and Function PLoS One March 2011 10.1371/journal.pone.0017673
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., Senescence-associated ribosome biogenesis defects contributes to cell cycle arrest through the Rb pathway. Nature cell biology June 2018
Cancer, Cell Cycle, Ser/Thr Kinases
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