TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro (1). Expression of TOPK is detected in male germ line progenitor cells, activated T-cells, and a variety of lymphomas and leukemias. In vitro studies have shown that activated TOPK phosphorylated p38 MAPK but not JNK or ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex and another unknown kinase, possibly Raf C or Raf A. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation or differentiation (2).
PBK, SPK, Nori-3, FLJ14385
1. Matsumoto, S. et al: Characterization of a MAPKK-like protein kinase TOPK. Biochem Biophys Res Commun. 2004; 325: 997-1004.
2. Simons-Evelyn, M. et al: PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells. Blood Cells Mol Dis, 2001; 27: 825-829.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Joon Kim Dong et al., Novel TOPK Inhibitor HI-TOPK-032 Effectively Suppresses Colon Cancer Growth Cancer Research June 2012 10.1158/0008-5472.CAN-11-3851
Hwan Park Jung et al., Phosphorylation of IKBα at Serine 32 by T-lymphokine-activated Killer Cell-originated Protein Kinase Is Essential for Chemoresistance against Doxorubicin in Cervical Cancer Cells Journal of Biological Chemistry February 2013 10.1074/jbc.M112.422170
A. Zykova Tatyana et al., T-LAK Cell-originated Protein Kinase (TOPK) Phosphorylation of Prx1 at Ser-32 Prevents UVB-induced Apoptosis in RPMI7951 Melanoma Cells through the Regulation of Prx1 Peroxidase Activity* Journal of Biological Chemistry September 2010 10.1074/jbc.M110.135905
G Gao et al., ADA-07 Suppresses Solar Ultraviolet-Induced Skin Carcinogenesis by Directly Inhibiting TOPK Mol Cancer Thera October 2016 10.1158/1535-7163.MCT-17-0212
Joon Kim Dong et al., CInQ-03, a novel allosteric MEK inhibitor, suppresses cancer growth in vitro and in vivo Carcinogenesis January 2013 10.1093/carcin/bgt015
Joon Kim Dong et al., (3-Chloroacetyl)-indole, a Novel Allosteric AKT Inhibitor, Suppresses Colon Cancer Growth In Vitro and In Vivo Cancer Prevention Research September 2011 10.1158/1940-6207.CAPR-11-0158
Rizkallah Raed et al., Identification of the oncogenic kinase TOPK/PBK as a master mitotic regulator of C2H2 zinc finger proteins Oncotarget January 2015 10.18632/oncotarget.2735
Cancer, Ser/Thr Kinases