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TOPK, Unactive

Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. T14-14G


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Catalog No. Pack Size Price (USD)
T14-14G-20 20 ug $215
T14-14G-50 50 ug $435
T14-14G-BULK BULK Contact Us  


Overview:

TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro (1). Expression of TOPK is detected in male germ line progenitor cells, activated T-cells, and a variety of lymphomas and leukemias. In vitro studies have shown that activated TOPK phosphorylated p38 MAPK but not JNK or ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex and another unknown kinase, possibly Raf C or Raf A. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation or differentiation (2).


Gene Aliases:

PBK, SPK, Nori-3, FLJ14385


Genbank Number:


References:


1. Matsumoto, S. et al: Characterization of a MAPKK-like protein kinase TOPK. Biochem Biophys Res Commun. 2004; 325: 997-1004.

2. Simons-Evelyn, M. et al: PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells. Blood Cells Mol Dis, 2001; 27: 825-829.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~68 kDa



Product Datasheets



RESEARCH AREAS

Cancer, Ser/Thr Kinases


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