Home    >   Primary Antibodies

Anti-PKC zeta

Rabbit Polyclonal Antibody
Catalog No. P75-63R


For enquiry on all Primary Antibodies email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
P75-63R-100 100 ug $270
P75-63R-BULK BULK Contact Us  


Overview:

PKCζ is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCζ also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCζ is an upstream modulator of p70S6K, an important regulator of cell proliferation (2).

References:


1. Kim, SJ. Et al: Phorbol ester effects in atypical protein kinase C zeta overexpressing NIH3T3 cells: possible evidence for crosstalk between protein kinase C isoforms. Biochem Biophys Res Commun. 1997 Aug 18;237(2):336-9.

2. Romanelli, A. et al: p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex. Mol Cell Biol. 1999 Apr;19(4):2921-8.


Specificity:

Recognizes the PKCζ protein


Cross Reactivity:

Western blot of human PKCz

PKCz from other species may also be detectable


Host:

Rabbit


Immunogen:

Synthetic peptide corresponding to amino acids 408-412 (TSTFC) of human PKCζ


Purification:

Affinity chromatography


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot with Anti-PKCζ (1:1000) using 3 μg, 10 μg, and 30 μg of 3T6 cell lysate.



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway


RELATED PRODUCTS


  PKC Lipid Activator, 10x, L51-39

  PKC nu, Active, P73-10G

  Anti-PKC theta, P74-63R

  Anti-PKC delta, P64-63CR


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox