Recombinant full-length human PKCiota was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. P68-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P68-10G-05 | 5 ug | $160 | |
P68-10G-10 | 10 ug | $226 | |
P68-10G-BULK | BULK | Contact Us |
Overview:
PKCι is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKCι showed greatest homology to PKCzeta, with 72% identity overall rising to 84% in the catalytic domain. Protein kinase C iota (PKCι) has been implicated in Ras signaling and is a critical downstream effector of oncogenic Ras in the colonic epithelium. Transgenic mice expressing constitutively active PKCι in the colon are highly susceptible to carcinogen-induced colon carcinogenesis (1).
Gene Aliases:
PRKCI; DXS1179E; MGC26534; nPKC-iota
Genbank Number:
References:
1. Murray, N R. et al: Protein kinase C iota is required for Ras transformation and colon carcinogenesis in vivo.
J Cell Biol. 2004 Mar 15;164(6):797-802.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~98 kDa
Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028
J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases
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