Rabbit Polyclonal Antibody
Catalog No. M02-652R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M02-652R-100 | 100 ul | $715 | |
M02-652R-BULK | BULK | Contact Us |
Overview:
MEK1 (MAP Kinase Kinase, also known as MKK) is a member of the dual specificity protein kinase family. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of the MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development (1). This pathway plays a key role in synaptic plasticity in the brain (2). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK 1 represents a likely target for pharmacological intervention in proliferative diseases such as cancer (3). MEK1 can be phosphorylated by MAP kinase at Thr292 and Thr386.
References:
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 1995 Jun;9(9):726-35.
2. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
3. Sebolt-Leopold, J S. et al: Blockade of the MAP Kinase Pathway Suppresses growth of colon tumors in vivo. Nature Med. 1999 5: 810-816.
Specificity:
Recognizes the MEK1/2 protein phosphorylated at serine 218 and serine 222
Cross Reactivity:
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus and Zebrafish
Host:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH
Purification:
Affinity Chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.
Spirli Carlo et al., Cyclic AMP/PKA-dependent paradoxical activation of Raf/MEK/ERK signaling in polycystin-2 defective mice treated with sorafenib Hepatology August 2012 10.1002/hep.25872
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
Chao; Xu, Fengrong; Niu, Yan; Wu, Yun; Sun, Jing; Peng Wang et al., Synthesis and Biological Evaluations of 3-Benzothiazol-2-yl Coumarin Derivatives as MEK1 Inhibitors Letters in Drug Design & Discovery October 2013 10.2174/15701808113109990012
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases
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