Mouse Monoclonal Antibody
Catalog No. S56-365BM
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S56-365BM-100 | 100 ug | $675 | |
S56-365BM-BULK | BULK | Contact Us |
Overview:
TAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).
References:
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.
Specificity:
Recognizes the mouse STAT5a protein phosphorylated at Tyrosine 694
Cross Reactivity:
Human, Mouse and Rat
Host Isotype / Clone#:
Mouse, IgG1
Immunogen:
The antibody was produced against synthesized peptide corresponding to residues surrounding Tyr694 of mouse STAT5a protein.
Purification:
Protein A chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Immunohistochemical analysis on formalin-fixed, paraffin embedded human breast tissues (40x) using Anti-Phospho-STAT5 (Tyr694) antibody.
Sample Data:
Western blot using Anti-phospho-STAT5 (Tyr694) antibody (1:1,000) shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1).
A. Cumaraswamy Abbarna et al., Nanomolar-Potency Small Molecule Inhibitor of STAT5 Protein ACS Medical Chemistry Letters September 2014 10.1021/ml500165r
Zeidan N et al., Magnetic bead-based electrochemical detection of interaction between epigallocatechin-3-gallate and STAT proteins Analytical Methods March 2015 10.1039/c5ay00169b
Zhanga Xiaolei et al., Orally bioavailable small-molecule inhibitor of transcription factor Stat3 regresses human breast and lung cancer xenografts PNAS January 2012 10.1073/pnas.1121606109
Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway
STAY CONNECTED
Fax: 1-604-232-4601