Specificity:

Recognizes the human SMAD1 protein phosphorylated at Serine 206

Cross Reactivity:

Human, Mouse, Rat and Dog

Host Isotype / Clone#:

Rabbit, IgG

Immunogen:

The antibody was produced against synthesized peptide corresponding to the region of amino acids containing Ser206 of the SMAD1 protein

Purification:

Immunoaffinity chromatography

Stability:

1yr at –20^oC from date of shipment

Sample Data:

Western blot using Affinity Purified Anti-Phospho-SMAD1 (Ser206) antibody (1:500 dilution) shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate.

References:

1. Hoodless, P. A. et al: MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 85: 489-500, 1996.

2. Nakashima, K. et al: Synergistic signaling in fetal brain by STAT3-Smad1 complex bridged by p300. Science 284: 479-482, 1999.