Recombinant human LOK (1-348) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. S29-11G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S29-11G-05 | 5 ug | $226 | |
S29-11G-10 | 10 ug | $325 | |
S29-11G-BULK | BULK | Contact Us |
Overview:
LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression (1). LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects (2).
Gene Aliases:
STK10, PRO2729
Genbank Number:
References:
1. Walter, S A. et al: Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue. J Biol Chem. 2003 May 16;278(20):18221-8.
2. Tao, L. et al: Opposing roles of serine/threonine kinases MEKK1 and LOK in regulating the CD28 responsive element in T-cells. Biochem J. 2002 Apr 1;363(Pt 1):175-82.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~65 kDa
Cardiovascular Disease, Ser/Thr Kinases
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