Native Swine MBP was extracted under acidic conditions and further purified by cation chromatography from pig brain (1). This product is routinely evaluated using active MAP Kinase 3/ERK1.
Catalog No. M42-51N
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MBP exists as four major forms in the pig CNS with apparent molecular weights of 21.5K, 20.2K, 18.5K, and 17.3K (2). Native pig MBP is extracted under acidic conditions and further purified by cation chromatography. MBP is an efficient substrate for numerous protein kinases including ERK/MAP Kinase family, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase (3, 4).
1. Chevalier D, et al. Purification of myelin basic protein from bovine brain. Protein Expr Purif. 18(2):229-34, 2000.
2. Sheng HZ, et al. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40.
3. Sanghera J. et al. Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk. FEBS Lett. 1990 Oct 29;273(1-2):223-6.
4. Martenson, et al., Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein. J. Biol. Chem. 258: 930, 1983.
Sample Protein comparison chart. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –20oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
C; Weerapana Wang et al., A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles Nature Methods May 2015 10.1038/nmeth.2759
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V Sharma et al., Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers. Elife February 2016 10.7554/eLife.11566
Brumbaugh Justin et al., NANOG Is Multiply Phosphorylated and Directly Modified by ERK2 and CDK1 In Vitro Stem Cell Reports January 2014 10.1016/j.stemcr.2013.12.005
Lee Rubin et al., METHODS, COMPOSITIONS AND KITS FOR PROMOTING MOTOR NEURON SURVIVAL AND TREATING AND DIAGNOSING NEURODEGENERATIVE DISORDERS Patent US 20160082015 April 2016
ERK/MAPK Pathway, Neurobiology