Recombinant human IKKβ (1-662) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. I03-10BG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
I03-10BG-05 | 5 ug | $226 | |
I03-10BG-10 | 10 ug | $325 | |
I03-10BG-BULK | BULK | Contact Us |
Overview:
IKKβ is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKβ is stimulated by TNF and IL1 and IKKβ forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKKβ blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKβ activity in vitro and in vivo by binding to IKKβ to reduce ATP binding (2).
Gene Aliases:
IKK2, IKBKB, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801
Genbank Number:
References:
1. Woronicz, J D. et al: IkappaB kinase-beta: NF-kappa-B activation and complex formation with IkappaB kinase-alpha and NIK. Science 278: 866-869, 1997.
2. Yin, M.-J. et al: The anti-inflammatory agents aspirin and salicylate inhibit the activity of I-kappa-B kinase-beta. Nature 396: 77-80, 1998.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~105 kDa
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