Recombinant human proteins expressed in Sf9 cells
Catalog No. C35-34G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C35-34G-20 | 20 ug | $215 | |
C35-34G-50 | 50 ug | $435 | |
C35-34G-BULK | BULK | Contact Us |
Overview:
CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2).
References:
1. Meyerson, M. et al: Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Molec. Cell. Biol. 14: 2077-2086, 1994.
2. Takaki, T. et al: Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro. J Biochem. 2005 Mar;137(3):381-6.
Alternative Name(s):
CDK6: PLSTIRE, MGC59692 CyclinD2: CCND2
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight:
CDK6 observed MW ~66 kDa. CDK6 calculated MW ~63 kDa. CyclinD2 observed MW ~60 kDa. CyclinD2 calculated MW ~59 kDa.
There are no related publications available for this product.
Cancer, Cell Cycle
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