Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M17-11G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M17-11G-05 | 5 ug | $226 | |
M17-11G-10 | 10 ug | $325 | |
M17-11G-BULK | BULK | Contact Us |
Overview:
MLK1 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway. The catalytic domain of MLK1 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MLK1 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini (1). MLK1 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation (2).
Gene Aliases:
MAP3K9, MEKK9; PRKE1
Genbank Number:
References:
1. Dorow, D S. et al: Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains. Europ. J. Biochem. 213: 701-710, 1993.
2. Durkin, J T. et al: Phosphoregulation of mixed-lineage kinase 1 activity by multiple phosphorylation in the activation loop. Biochemistry. 2004 Dec 28;43(51):16348-55.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~77 kDa
Das S et al., Mixed lineage kinase 3 promotes breast tumorigenesis via phosphorylation and activation of p21-activated kinase 1. Oncogene January 2019 10.1038/s41388-019-0690-0
et al., Ripretinib (DCC-2618) Is a Switch Control Kinase Inhibitor of a Broad Spectrum of Oncogenic and Drug-Resistant KIT and PDGFRA Variants. Cancer cell February 2020
Apoptosis/Autophagy, Cell Cycle, JNK/SAPK Pathway, Ser/Thr Kinases
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