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Recombinant Achromobacter lyticus Lys-c (lysyl-endo-peptidase) (206-473aa) was expressed in E. coli cells using a C-terminal His tag.
Catalog No. L585-31HP
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L585-31HP-20 | 20 ug | $165 | |
L585-31HP-500 | 500 ug | $1105 | |
L585-31HP-01 | 1 mg | $1607 | |
L585-31HP-BULK | BULK | Contact Us |
Overview:
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 – 37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.
Genbank Number:
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Alternative Name(s):
API, Protease I, Lysyl endopeptidase
Reconstitution Protocol:
Add distilled H2O to a final concentration of 1 mg/ml.
Storage and Stability:
Store product at –20°C. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.
Digestion Conditions:
Catalytic pH range: 9.0 ~ 9.5 Catalytic temperature range: 30 ~ 37oC
There are no related publications available for this product.
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