Recombinant full-length human LYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. L13-18G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L13-18G-05 | 5 ug | $226 | |
L13-18G-10 | 10 ug | $325 | |
L13-18G-BULK | BULK | Contact Us |
Overview:
LYN A is a member of the Src-family of protein kinases that participate in signaling pathways of a variety of cell surface receptors and are localized to the cytoplasmic side of the plasma membrane (1). LYN A is biosynthetically transported to the plasma membrane via the Golgi pool of caveolin along the secretory pathway. The trafficking of LYN A from the Golgi apparatus to the plasma membrane is inhibited by deletion of the kinase domain but not by kinase inactivation. The kinase domain of LYN A plays a role in LYN A trafficking besides catalysis of substrate phosphorylation. cDNA microarray analysis revealed that LYN A may be involved in the changes that are related to tumor pathogenesis (2).
Gene Aliases:
JTK8, FLJ26625
Genbank Number:
References:
1. Kasahara, K. et al: Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain. J. Cell Biol., 2004;165(5):641-52.
2. Baran, C.P. et al: The inositol 5'-phosphatase SHIP-1 and the Src kinase Lyn negatively regulate macrophage colony stimulating factor-induced Akt activity. J. Biol. Chem. 2003; 278(40):38628-36
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~81 kDa
Huang Yongqi et al., The Activity and Stability of the Intrinsically Disordered Cip/Kip Protein Family AreRegulated by Non-Receptor Tyrosine Kinases Journal of Molecular Biology January 2015 10.1016/j.jmb.2014.11.011
Z. Wang Gary et al., Regulation of Yin Yang 1 by Tyrosine Phosphorylation Journal of Biological Chemistry September 2015 10.1074/jbc.M115.660621
E. Salhia Hussam et al., Cardiac troponin I tyrosine 26 phosphorylation decreases myofilament Ca 2+ sensitivity and accelerates deactivation Journal of Molecular and Cellular Cardiology September 2014 10.1016/j.yjmcc.2014.09.013
Abe N et al., Bisorbicillinol inhibits Lyn tyrosine kinase for allergic response on RBL-2H3 cells Bioorganic & Medicinal Chemistry Letters March 2019 10.1016/j.bmcl.2019.01.019
Lia Feng et al., Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry Journal of Chromatography A June 2015 10.1016/j.chroma.2015.04.055
AKT/PKB Pathway, Cancer, Cardiovascular Disease, Cellular Stress, Cytoplasmic Tyrosine Kinases, Inflammation
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