Recombinant Human GM-CSF (CSF2) was expressed in E. coli cells.
Catalog No. G806-40N
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
G806-40N-20 | 20 ug | $314 | |
G806-40N-100 | 100 ug | $985 | |
G806-40N-BULK | BULK | Contact Us |
Overview:
GM-CSF is a hematopoietic growth factor that is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes following their activation by antigens or mitogens. GM-CSF stimulates the development of macrophages and neutrophils and plays a role in the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo compared to its non-glycosylated counterpart. Human and murine GM-CSF are species-specific and therefore, exhibit no cross-reactivity. Recombinant Human GM-CSF is a non-glycosylated, monomeric protein that contains intra-chain disulfide bonds.
Gene Aliases:
GM-CSF, Granulocyte-macrophage colony-stimulating factor, CSF-2, Pluripoietin-alpha, Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
15.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be ≤ 0.5 ng/mL
Purity:
Recombinant Human GM-CSF (CSF2) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
There are no related publications available for this product.
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