Recombinant human ROS1 (F2004I) (1881-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. R14-12GG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
R14-12GG-05 | 5 ug | $226 | |
R14-12GG-10 | 10 ug | $325 | |
R14-12GG-BULK | BULK | Contact Us |
Overview:
ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes. ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor. The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines (1). The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase. Direct interaction of ROS1 and the SHP-1 phosphatase can lead to efficient downregulation of ROS1-mediated signaling (2). Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain. Structural models of ROS1 (F2004I) predict resistance to crizotinib, entrectinib, and lolartanib via steric hindrance (3).
Genbank Number:
References:
1. Charest, A. et al: Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Genes Chromosomes Cancer 37: 58-71, 2003.
2. Biscup, C. et al: Visualization of SHP-1-target interaction. J Cell Sci. 2004 Oct 1;117(Pt 21):5165-78.
3. Gendarme, S. et al: ROS-1 Fusions in Non-Small-Cell Lung Cancer: Evidence to Date. Curr. Oncol. 29(2):641-658, 2022.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Alternative Name(s):
c-ros-1; MCF3; ROS
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
Observed MW ~82 kDa. Calculated MW ~80 kDa.
There are no related publications available for this product.
Cancer, Cell Cycle
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