Recombinant mouse IKKbeta (1-662) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. I03M-10BG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
I03M-10BG-05 | 5 ug | $226 | |
I03M-10BG-10 | 10 ug | $325 | |
I03M-10BG-BULK | BULK | Contact Us |
Overview:
IKK beta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKK beta is stimulated by TNF and IL1 and IKK beta forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKK beta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKK beta activity in vitro and in vivo by binding to IKK beta to reduce ATP binding (2).
Gene Aliases:
IKK-2; IKK-beta; IKK2; IKKbeta; IKK[b]; IKBKB; AI132552
Genbank Number:
References:
1. Woronicz, J D. et al: IkappaB kinase-beta: NF-kappa-B activation and complex formation with IkappaB kinase-alpha and NIK. Science 278: 866-869, 1997.
2. Yin, M.-J. et al: The anti-inflammatory agents aspirin and salicylate inhibit the activity of I-kappa-B kinase-beta. Nature 396: 77-80, 1998.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
105 kDa
Moa Min et al., Phosphorylation of Xenopus p31comet potentiates mitotic checkpoint exit Cell Cycle April 2015 10.1080/15384101.2015.1033590
Hee Park Sun et al., Regulation of anoikis by deleted in breast cancer-1 (DBC1) through NF-KB Apoptosis : an International Journal on Programmed Cell Death April 2013 10.1007/s10495-013-0847-1
AKT/PKB Pathway, Apoptosis/Autophagy, Inflammation, p38 Pathway
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