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PAD Cocktail, Active

The five full length recombined PAD proteins (1, 2, 3, 4 and 6) expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. This mixture is ideally suited for in vitro citrullination of protein targets.
Catalog No. P312-37C


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Catalog No. Pack Size Price (USD)
P312-37C-25 25 ug $325
P312-37C-100 100 ug $809
P312-37C-BULK BULK Contact Us  


Overview:

The PAD family of enzymes is responsible for the catalysis of arginine-to-citrulline conversion in target proteins. There are 5 mammalian PAD isoforms (PAD1-4,6) that share ~50% sequence homology. Each PAD enzyme has its own typical subcellular localization, tissue distribution and substrate specificity, although no substrate protein has been found so far for PAD6.


References:


1. Tilvawala, R et. Al: Peptidyl arginine deiminases: detection and finctional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11, 2019.

2. Clancy K.W. et al., Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol., 30, 1-6, 2016.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

SDS-PAGE image of PAD Cocktail Proteins. The five PAD isoform proteins from left to right are: PAD1, PAD4, PAD3, PAD6 and PAD2. The far-right lane contains PAD Cocktail.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

PAD1: ~95 kDa
PAD2: ~99 kDa
PAD3: ~97 kDa
PAD4: ~96 kDa
PAD6: ~100 kDa



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Inflammation


RELATED PRODUCTS


  PAD4, Active, P312-310DG

  PAD6 Protein, P312-30FG

  PAD1 Inhibitor Screening Kit, P310G-863

  PAD2 (mouse) Inhibitor Screening Kit, P310BG-863


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