Recombinant human fusion KIAA1549 (1320-1749 exon16) -BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. B08-19BG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
B08-19BG-05 | 5 ug | $259 | |
B08-19BG-10 | 10 ug | $380 | |
B08-19BG-BULK | BULK | Contact Us |
Overview:
KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0 Mb tandem duplication in chromosome band 7q34 (1). The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers (2).
Gene Aliases:
KIAA1549: none
BRAF: BRAF1, RAFB1, B-raf, MGC126806, MGC138284
Genbank Number:
References:
1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12(7):621-30.
2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22(6):841-7.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~125 kDa
Wua Zeng et al., Design, synthesis and biological evaluation of indole derivatives as novel inhibitors targeting B-Raf kinase Chinese Chemical Letters February 2014 10.1016/j.bmcl.2012.05.053
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Angiogenesis, Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, JAK/STAT Pathway, Ser/Thr Kinases
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