Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. L03-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L03-10G-05 | 5 ug | $226 | |
L03-10G-10 | 10 ug | $325 | |
L03-10G-BULK | BULK | Contact Us |
Overview:
LCK (p56lck) is a member of the src family of non-receptor tyrosine kinases. It was identified as a gene rearranged and overexpressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene (1). Lck behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show overexpression of LCK, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers (2).
Gene Aliases:
YT16, p56lck, pp58lck
Genbank Number:
References:
1. Fischer, S. et al: The amino terminal region of the p56 lck from LSTRA exerts negative modulation on the tyrosine kinase activity. Biochem Biophys Res Commun. 1987 Mar 30;143(3):819-26.
2. Veillette, A. et al: Expression of the lck tyrosine kinase gene in human colon carcinoma and other non-lymphoid human tumor cell lines. Oncogene Res. 1987 Sep-Oct;1(4):357-74.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~84 kDa
Huang Yongqi et al., The Activity and Stability of the Intrinsically Disordered Cip/Kip Protein Family AreRegulated by Non-Receptor Tyrosine Kinases Journal of Molecular Biology January 2015 10.1016/j.jmb.2014.11.011
Deng Yang et al., Global Analysis of Human Nonreceptor Tyrosine Kinase Specificity Using High-Density Peptide Microarrays Journal of Proteome Research August 2014 10.1021/pr500503q
Cancer, Cytoplasmic Tyrosine Kinases, Inflammation, JAK/STAT Pathway, NfkB Pathway
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