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Anti-PAK1

Rabbit Polyclonal Antibody
Catalog No. P02-63R


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Catalog No. Pack Size Price (USD)
P02-63R-100 100 ug $347
P02-63R-BULK BULK Contact Us  


Overview:

PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2).

References:


1. Jaffer, Z M. et al: p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 2002 Jul;34(7):713-7.

2. Parrini, M C. et al: Spatiotemporal regulation of the Pak1 kinase. Biochem Soc Trans. 2005 Aug;33(Pt 4):646-8.


Specificity:

Recognizes the PAK1 protein


Cross Reactivity:

Western blot PAK1 from human and mouse cells

PAK1 from other species may also be detectable


Host:

Rabbit


Immunogen:

Synthetic peptide corresponding to amino acids 210-214 of human PAK1


Purification:

Affinity chromatography


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot with Anti-PAK1 (1:1000) using 3 μg, 10 μg, and 30 μg of Jurkat cel lysate.


Sample Data:

Representative western blot with Anti-PAK1 (1:1000) using 20 ng of human recombinant PAK1, PAK2, PAK3, PAK4, and PAK7.



Product Datasheets



RESEARCH AREAS

Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases


RELATED PRODUCTS


  PAK1/CDC42, Active, P02-10G

  PAKtide, P08-58

  PAK4, Active, P05-10BG

  PAK2 Protein, P03-34G


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