Rabbit Polyclonal Antibody
Catalog No. M02-65R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M02-65R-100 | 100 ug | $325 | |
M02-65R-BULK | BULK | Contact Us |
Overview:
MEK1 (MAP Kinase Kinase, MKK) is a member of the dual specificity protein kinase family. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of the MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development (1). This pathway plays a key role in synaptic plasticity in the brain (2). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK 1 represents a likely target for pharmacological intervention in proliferative diseases such as cancer (3). Ser222 is essential for the kinase activity of MEK1 (4).
References:
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 1995 Jun;9(9):726-35.
2. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
3. Sebolt-Leopold, J S. et al: Blockade of the MAP Kinase Pathway Suppresses growth of colon tumors in vivo. Nature Med. 1999 5: 810-816.
4. Pagès G. et al: Co nstitutive mutant and putative regulatory serine phosphorylation site of mammalian MAP kinase kinase (MEK1). EMBO J. 1994 Jul 1;13(13):3003-10.
Specificity:
Recognizes the MEK1/2 protein phosphorylated at serine 222
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of extracts from Jurkat cells using MEK1 antibody (lanes 1 and 2) and anti-phospho-MEK1/2 (Ser222) antibody (lanes 3 and 4).
Sample Data:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-MEK1/2 (Ser222) antibody.
Spirli Carlo et al., Cyclic AMP/PKA-dependent paradoxical activation of Raf/MEK/ERK signaling in polycystin-2 defective mice treated with sorafenib Hepatology August 2012 10.1002/hep.25872
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
Chao; Xu, Fengrong; Niu, Yan; Wu, Yun; Sun, Jing; Peng Wang et al., Synthesis and Biological Evaluations of 3-Benzothiazol-2-yl Coumarin Derivatives as MEK1 Inhibitors Letters in Drug Design & Discovery October 2013 10.2174/15701808113109990012
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases
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