Recombinant full-length tag-free human CSK was expressed by baculovirus in Sf9 insect cells.
Catalog No. C63-10U
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C63-10U-05 | 5 ug | $226 | |
C63-10U-10 | 10 ug | $325 | |
C63-10U-BULK | BULK | Contact Us |
Overview:
CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src through tyrosine phosphorylation of the c-src carboxy terminus (1). A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells.
Gene Aliases:
MGC117393
Genbank Number:
References:
1. Partanen, J. et al: Cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416-src). Oncogene 6: 2013-2018, 1991.
2. Cloutier, J.-F. et al: Association of inhibitory tyrosine protein kinase p50(csk) with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells. EMBO J. 15: 4909-4918, 1996.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~52 kDa
Huang Yongqi et al., The Activity and Stability of the Intrinsically Disordered Cip/Kip Protein Family AreRegulated by Non-Receptor Tyrosine Kinases Journal of Molecular Biology January 2015 10.1016/j.jmb.2014.11.011
Roglin Lars et al., DNA and RNA-Controlled Switching of Protein Kinase Activity ChemBioChem March 2009 10.1002/cbic.200800771
Cancer, Cytoplasmic Tyrosine Kinases, Inflammation
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