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Cas13a (LwC2c2), Active

Recombinant Leptotrichia wadei Cas13a (LwC2c2) (1-1152) was expressed in E. coli with a C-terminal His tag.
Catalog No. CS13A-E321H


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Catalog No. Pack Size Price (USD)
CS13A-E321H-50 50 pmol $180
CS13A-E321H-100 100 pmol $295
CS13A-E321H-200 200 pmol $525
CS13A-E321H-1000 1000 pmol $1395
CS13A-E321H-BULK BULK Contact Us  


Overview:

Cas13a is RNA-guided RNA endonuclease that belongs to class 2 type VI CRISPR-Cas system (1). Cas13a possess both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner. This property of Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection diagnostic tool for clinical samples. Diagnostics using Cas13a for detection of SARS-CoV-2 offers promising alternative to quantitative PCR (3). In addition, CRISPR-Cas13a system provides opportunity for the development of clinical research in precision oncology (4).


Gene Aliases:

LwCas13a, LwaCas13a, LwC2c2, EndoRNase, CRISPR-associated endoribonuclease Cas13a


Genbank Number:


References:


1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270–3.

2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573.

3. Patchsung, M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x.

4. Zhang J, You Y. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 2020, 17(1):6-8.


Activity:

Sample activity data. For lot-specific data, please refer to the specific datasheet.


Purity :

Sample purity data. For lot-specific data, please refer to specific datasheets.


Molecular Weight:

Approx 130 kDa


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Acute Respiratory Distress Syndrome , Cardiovascular Disease, Cellular Stress, COVID19, Gastrointestinal Diseases , Infectious Diseases , Inflammation, Lung Diseases , Metabolic Disorder, Neurobiology, severe acute respiratory syndrome coronavirus 2 , Virology


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