Home   >    Enzymes   >   Other Enzymes

Immobilized Lys-c, Silica

Recombinant tag-free Lys-c (Lysyl-Endopeptidase) was expressed in E. coli cells.

Matrix: cross-linked silica Average Particle Size: 200 μm Ligand Density: 5 mg protein / ml matrix Maximum Flow Rate, Pressure: 300 cm/h, 2 bar
Catalog No. L585-31SN


Shop Online



Catalog No. Pack Size Price (USD)
L585-31SN-500 500 mg Add to Cart
L585-31SN-BULK BULK Contact Us  


Overview:

Silica immobilized proteases are amenable to large-scale proteolysis processes. Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 – 37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.

Immobilized Lysyl-Endopeptidase is covalently cross-linked to a silica matrix circumventing the need for enzyme removal after cleavage. The resin can be reused ~20 times after regenerating when proper storage conditions are followed.


Gene Aliases:

API, Protease I, Lysyl endopeptidase



Sample Data:


Enzyme Commission Number:

EC 3.4.21.50


Storage, Stability, and Shipping:

Store product at 2 - 4oC for up to 6 months.


Digestion Conditions:

Catalytic pH Range: 8.0 ~ 9.5
Catalytic Temperature Range: 15 ~ 37oC
Enzyme : Substrate Ratio: 1:200 ~ 1:2,000



Product Datasheets



There are no related publications available for this product.


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox