Specificity:

Recognizes proteins with tri-methylation on lysine residues (N-epsilon).

Cross Reactivity:

Pan-specific antibody. No reaction with acetylated, mono-methylated and di-methylated proteins.

Host:

Rabbit

Immunogen:

Methylated lysine-KLH conjugates.

Purification:

By affinity chromatography on a tri-methyl lysine agarose column

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri-ε-N-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional N-ε-tri-methyl lysine peptide inhibitor (50 ng/μl) (B).

Sample Data:

Immunohistochemistry analysis of human tissue with Anti-Methylated Lysine (Tri-ε-N-methyl) (1:100).