Recombinant full-length human p110 delta and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Catalog No. P30-102H
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PI3K (p110 delta/p85 alpha) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110 delta/p85 alpha) plays a role in oncogenic transformation, and is consistently expressed at a high level in blast cells from AML. PI3K (p110 delta/p85 alpha) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110 delta/p85 alpha) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
p110 delta: p110 delta p85 alpha: PIK3R1, GRB1, p85-ALPHA
1. Vanhaesebroeck, B. et.al: p110-delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Nat. Acad. Sci. 94: 4330-4335, 1997.
2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
p110 delta~111 kDa and p85 alpha~86 kDa
Y Zheng et al., Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Nature July 2013 10.1038/nature12308
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
He Chenchen et al., Characterization of a novel p110β-specific inhibitor BL140 that overcomes MDV3100-resistance in castration-resistant prostate cancer cells Prostate August 2017 10.1002/pros.23377
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling