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SpCas9, Active

Recombinant full-length Streptococcus pyogenes Cas9 was expressed in E. coli with a C-terminal His tag.
Catalog No. CS09-E311H


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Catalog No. Pack Size Price (USD)
CS09-E311H-50 50 ug $180
CS09-E311H-100 100 ug $270
CS09-E311H-200 200 ug $405
CS09-E311H-500 500 ug $708
CS09-E311H-BULK BULK Contact Us  


Overview:

Streptococcus pyogenes Cas9 is a type II-A Cas9 protein and is the most used in most current CRISPR-based genetic engineering methodologies (1). CRIPSR RNAs (crRNAs) fused to trans-activating CRISPR RNAs (tracrRNA) direct sequence-specific cleavage by Cas9, introducing double-stranded breaks in cell-invading DNA in bacterial and archaeal CRIPSR/Cas9 systems (1,2). Cas9 requires the 5’-NGG-3’ protospacer adjacent motif (PAM) to destabilize the target DNA (3). Cas9’s HNH endonuclease domain cleaves the strand complementary to the crRNA while its RuvC endonuclease domain cleaves the non-complementary strand (2).


Gene Aliases:

Cas9, Csn1, Spy_1046


Genbank Number:


References:

1. Jinek, M. et al. Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation. Science. 2014, 343(6176): 1247997. 2. Jinek, M. et al. A programmable dual RNA-guided endonuclease in adaptive bacterial immunity. Science. 2012, 337(6096): 816-821. 3. Anders, C. et al. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature. 2014, 513(7519): 569-573.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet


Purity:

Sample Purity graph. For specific information on a given lot, see related technical data sheet


Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

Approx 156kDa



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cell Cycle


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