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AsCas12a, Active

Recombinant Acidaminococcus AsCas12a was expressed in E. coli with a C-terminal His tag.
Catalog No. CS12A-E311H


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Catalog No. Pack Size Price (USD)
CS12A-E311H-50 50 pmol $180
CS12A-E311H-100 100 pmol $295
CS12A-E311H-200 200 pmol $525
CS12A-E311H-1000 1000 pmol $1395
CS12A-E311H-BULK BULK Contact Us  


Overview:

CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AsCas12a is a CRISPR-associated endonuclease from Acidaminococcus and it can be engineered to achieve combinatorial genetic screening with high efficiency (2). Engineered AsCas12a further enables accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells and helps fight against sickle cell disease (4).


Gene Aliases:

Cpf1, AsCpf1, CRISPR-associated endonuclease Cpf1


Genbank Number:


References:

1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170. 2. Morange, M. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223. 3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9. 4. DeLong, R. Ushering in a new era of RNA-based therapies. Commun Biol. 2021. doi: https://doi.org/10.1038/s42003-021-02150-w


Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

130 kDa



Product Datasheets



There are no related publications available for this product.


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