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LbuCas13a, Active

Recombinant tag-free Leptotrichia buccalis LbuCas13a was expressed in E. coli cells.
Catalog No. CS13A-E322U


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Catalog No. Pack Size Price (USD)
CS13A-E322U-50 50 pmol $180
CS13A-E322U-100 100 pmol $295
CS13A-E322U-200 200 pmol $525
CS13A-E322U-1000 1000 pmol $1395
CS13A-E322U-BULK BULK Contact Us  


Overview:

Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).

Genbank Number:


References:

1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270–3. 2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573. 3. Patchsung M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x. 4. Charles E, et al: Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression. bioRxiv. doi: https://doi.org/10.1101/2021.05.26.445687.


Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Purity:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.


Molecular weight:

132kDa


Specific Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet



Product Datasheets



There are no related publications available for this product.


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