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TtCsm6, Active

Recombinant Thermus thermophilus TtCsm6 was expressed in E. coli cells using a C-terminal His tag.
Catalog No. CS06-E311H


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Catalog No. Pack Size Price (USD)
CS06-E311H-50 50 pmol $180
CS06-E311H-100 100 pmol $295
CS06-E311H-200 200 pmol $525
CS06-E311H-1000 1000 pmol $1395
CS06-E311H-BULK BULK Contact Us  


Overview:

TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).


Gene Aliases:

CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6, csm6


Genbank Number:


References:

1. Niewoehner, O. et al: Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. Rna 22(3):318-329,2016. 2. Niewoehner, O. et al: Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548, 2017.


Specific Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.


Purity:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.


Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular weight:

MW 53kDa



Product Datasheets



There are no related publications available for this product.


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