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Cas12 (LbCpf1), Active

Recombinant Lachnospiraceae Cas12 (LbCpf1) (1-1227) was expressed in E. coli with a N-terminal GST tag.
Catalog No. C12CR-E241G


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Catalog No. Pack Size Price (USD)
C12CR-E241G-50 50 pmol $180
C12CR-E241G-100 100 pmol $295
C12CR-E241G-200 200 pmol $525
C12CR-E241G-1000 1000 pmol $1395
C12CR-E241G-BULK BULK Contact Us  


Overview:

CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing the disease spread. Several CRISPR-Cas12 based SARS-CoV-2 detection assays have been developed (4,5). CRISPR/Cas12 (LbCpf1) is a Cas12 synthetic construct derived from Lachnospiraceae bacterium.


Gene Aliases:

Cpf1 (synthetic construct)


Genbank Number:


References:

1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.

2. Morange, M. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.

3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9.

4. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: https://doi.org/10.1101/ 2020.03.06.20032334.

5. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/ 2020.02.29.971127.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.


Purity:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.


Molecular Weight:

150 kDa (calculated MW ~164 kDa)



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cardiovascular Disease, Cell Cycle, COVID19, Gastrointestinal Diseases , Infectious Diseases , Inflammation, Invasion/Metastasis, Lung Diseases , Metabolic Disorder, Neurobiology, severe acute respiratory syndrome coronavirus 2


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