Rabbit Polyclonal Antibody
Catalog No. S22-635R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S22-635R-100 | 100 ul | $795 | |
S22-635R-500 | 500 ul | $2150 | |
S22-635R-BULK | BULK | Contact Us |
Overview:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Alpha synuclein phosphorylated at serine 129 constitutes 90% of the alpha synuclein found in Lewy bodies (7, 8).
References:
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
7. Fujiwara H., et al. (2002) Nat Cell Biol. 4(2):160-4.
8. Anderson J.P., et al. (2006) J Biol Chem. 281(40):29739-52.
Specificity:
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Cross Reactivity:
Human, Mouse, Rat
Host:
Rabbit
Immunogen:
Synthetic peptide of Human Alpha Synuclein pSer129 (40-140aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Stability:
1yr at –20⁰C from date of shipment. Avoid freeze/thaw cycle.
Sample Data:
Western Blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Alpha Synuclein pSer129 Polyclonal Antibody (Cat# S22-635R). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Human Alpha Synuclein Monomer (0.5 µg). Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30, 16 kDa.
Sample Data:
Immunohistochemistry analysis using Rabbit Alpha Synuclein pSer129 Polyclonal Antibody (Cat# S22-635R). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Alpha Synuclein pSer129 Polyclonal Antibody (Cat# S22-635R) at 1:50 for 40 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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