Rabbit Polyclonal Antibody
Catalog No. P74-63R
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P74-63R-100 | 100 ug | $347 | |
| P74-63R-BULK | BULK | Contact Us |
Overview:
Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).
References:
1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fasligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.
2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.
Specificity:
Recognizes the PKCθ protein
Cross Reactivity:
Western blot human PKCtheta
PKCdelta/theta from other species may also be detectable.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to amino acids 674-678 of human PKCθ
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti- PKCθ (1:1000) using 20ng of GST –tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ, and PKCι.
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Chia Chuang Huai et al., The kinase GLK controls autoimmunity and NF-KB signaling by activating the kinase PKC-θ in T cells Nature Immunology October 2011 10.1038/ni.2121
Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028
J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245
Tokugawa Seiji et al., The expression pattern of PKCθ in satellite cells of normal and regenerating muscle in the rat Neuropathology October 2008 10.1111/j.1440-1789.2008.00967.x
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway
PKC gamma, Active, P66-10G
PKC alpha, Active, P61-18G
PKC iota, Active, P68-10G
PKC mu, Active, P72-10G
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