Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. Z01-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
Z01-10G-05 | 5 ug | $226 | |
Z01-10G-10 | 10 ug | $325 | |
Z01-10G-BULK | BULK | Contact Us |
Overview:
ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest. The activity of ZAK plays a role in cell cycle checkpoint regulation as well as being involved in regulating actin organization (1). Expression of kinase-dead ZAK in mouse fibroblasts disrupts actin stress fibers and causes morphologic changes. ZAK can activate JNK through MKK4/MKK7 and ERK5/p38-gamma via MKK3/MKK6. Expression of ZAK increases the population of cells in the G2/M phase of the cell cycle, whereas dominant-negative ZAK attenuated the G2 arrest caused by gamma radiation (2).
Gene Aliases:
AZK, MLT, MRK, MLK7, MLTK, mlklak, MAP3K20
Genbank Number:
References:
1. Yang J-J, et al: Mixed lineage kinase ZAK utilizing MKK7 and not MKK4 to activate the c-Jun N-terminal kinase and playing a role in the cell arrest. Biochem. Biophys. Res. Commun. 297: 105-110, 2002.
2. Gross E. A, et al: MRK, a mixed lineage kinase-related molecule that plays a role in gamma-radiation-induced cell cycle arrest. J. Biol. Chem. 277: 13873-13882, 2002
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~82 kDa
Apoptosis/Autophagy, Cancer, Cell Cycle, ERK/MAPK Pathway, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases
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