Rabbit Polyclonal Antibody
Catalog No. L95-67R
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Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on lysine residues is one example of post-translational modification and is performed by a variety of protein methyl transferases in the cell. C/EBPs are extensively modified by methylation of lysine side chains and this regulated methylation profoundly affects the activity of C/EBPs (1). The Methylated Lysine antibody detects methylation on lysine residues in proteins and peptide.
1. Leutz, R. et al: Crosstalk between phosphorylation and multi-site arginine/lysine methylation in C/EBPs. Transcr. 2011 Jan;2(1):3-8.
Recognizes proteins with tri-methylation on lysine residues (N-epsilon).
Pan-specific antibody. No reaction with acetylated, mono-methylated and di-methylated proteins.
Methylated lysine-KLH conjugates.
By affinity chromatography on a tri-methyl lysine agarose column
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri-ε-N-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional N-ε-tri-methyl lysine peptide inhibitor (50 ng/μl) (B).
Immunohistochemistry analysis of human tissue with Anti-Methylated Lysine (Tri-ε-N-methyl) (1:100).
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