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Anti-Methylated Arginine (NG-Mono-methyl)

Rabbit Polyclonal Antibody
Catalog No. A95-67R


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Catalog No. Pack Size Price (USD)
A95-67R-100 100 ug $380
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Overview:

Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on Arginine residues is one example of post-translational modification and is performed by a variety of protein methyl transferases in the cell. Arginine methylation of Rad9 for example is performed by protein arginine methyltransferase 5 (PRMT5) is important for regulation of Rad9 function and is a major element for maintaining genome integrity (1). The Methylated Arginine antibody detects Methylation on Arginine residues in proteins and peptide.

References:


1. He, W. et al: A role for the arginine methylation of Rad9 in checkpoint control and cellular sensitivity to DNA damage. Nucleic Acids Res. 2011 Feb 14.


Specificity:

Recognizes proteins with NG-mono-methylated arginine residues.


Cross Reactivity:

Pan-specific antibody. No reaction to acetylated proteins, di-methylated and tri-methylated proteins.


Host:

Rabbit


Immunogen:

NG-mono-methylated arginine-KLH conjugates


Purification:

By affinity chromatography on a mono-methyl arginine agarose column


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot of the mono-methylated arginine protein profile with Anti-Methylated Arginine (NG-Mono-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional mono-methyl arginine peptide inhibitor (B).


Sample Data:

Immunofluorecent stain of human melanoma (MMRU) cell with Anti-Methylated Arginine (NG-Mono-methyl).



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Apoptosis/Autophagy, Cancer, Cell Cycle


RELATED PRODUCTS


  Anti-Methylated Arginine (NG, NG-Di-methyl), A96-67R


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