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Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated

Rabbit Polyclonal Antibody
Catalog No. L95-67DR


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Catalog No. Pack Size Price (USD)
L95-67DR-100 100 ug $380
L95-67DR-BULK BULK Contact Us  


Overview:

Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on lysine residues is one example of post-translational modification and is performed by a variety of protein methyltransferases in the cell. C/EBPs are extensively modified by methylation of lysine side chains and this regulated methylation profoundly affects the activity of C/EBPs (1). The Methylated Lysine Antibody conjugated to HRP detects methylation on lysine residues in proteins and peptides.

References:


1. Leutz, R. et al: Crosstalk between phosphorylation and multi-site arginine/lysine methylation in C/EBPs. Transcr. 2011 Jan;2(1):3-8.


Specificity:

Recognizes proteins with tri-methylation on lysine residues (N-epsilon).


Cross Reactivity:

Pan-specific antibody. No cross-reactions detected with acetylated, mono-methylated and di-methylated proteins.


Host:

Rabbit


Immunogen:

Methylated lysine-KLH conjugates.


Purification:

By affinity chromatography on a tri-methyl lysine agarose column


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated (1:1000) using human melanoma cells (MMRU) lysate (A) and MMRU lysate plus additional N- epsilon-tri-methyl lysine peptide inhibitor (50 ng/ul) (B).



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology


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